diff --git a/content/applications/app_specific/bioinformatics_tools/de_novo_assembly_tools/trinity/_index.md b/content/applications/app_specific/bioinformatics_tools/de_novo_assembly_tools/trinity/_index.md index 59165f19a38f3c350bacb29c83239352ff322e95..6e19c01b92c0ca5ce254080247c7e0e73c9154b3 100644 --- a/content/applications/app_specific/bioinformatics_tools/de_novo_assembly_tools/trinity/_index.md +++ b/content/applications/app_specific/bioinformatics_tools/de_novo_assembly_tools/trinity/_index.md @@ -5,7 +5,7 @@ weight = "52" +++ -[Trinity](https://github.com/trinityrnaseq/trinityrnaseq/wiki) is a method for efficient and robust de novo reconstruction of transcriptomes from RNA-Seq data. Trinity combines three independent software modules: `Inchworm`, `Chrysalis`, and `Butterfly`. All these modules can be applied sequentially to process large RNA-Seq datasets. +[Trinity](https://github.com/trinityrnaseq/trinityrnaseq/wiki) is a method for efficient and robust de novo reconstruction of transcriptomes from RNA-Seq data. Trinity combines four independent software modules: `Normalization`, `Inchworm`, `Chrysalis` and `Assembly`. All these modules can be applied sequentially to process large RNA-Seq datasets. The basic usage of Trinity is: @@ -24,25 +24,25 @@ Running the Trinity pipeline from beginning to end on large datasets may exceed {{% panel theme="info" header="Step 1 Options" %}} {{< highlight bash >}} -Trinity.pl [options] --no_run_chrysalis +Trinity [options] --no_run_inchworm {{< /highlight >}} {{% /panel %}} {{% panel theme="info" header="Step 2 Options" %}} {{< highlight bash >}} -Trinity.pl [options] --no_run_quantifygraph +Trinity [options] --no_run_chrysalis {{< /highlight >}} {{% /panel %}} {{% panel theme="info" header="Step 3 Options" %}} {{< highlight bash >}} -Trinity.pl [options] --no_run_butterfly +Trinity [options] --no_distributed_trinity_exec {{< /highlight >}} {{% /panel %}} {{% panel theme="info" header="Step 4 Options" %}} {{< highlight bash >}} -Trinity.pl [options] +Trinity [options] {{< /highlight >}} {{% /panel %}} diff --git a/content/applications/app_specific/bioinformatics_tools/de_novo_assembly_tools/trinity/running_trinity_in_multiple_steps.md b/content/applications/app_specific/bioinformatics_tools/de_novo_assembly_tools/trinity/running_trinity_in_multiple_steps.md index 5bce95bb9433d0a3b2a315781c477699be245ae2..086a9ed4b3fc71fdaadf95fa86f370ed8dce7a4b 100644 --- a/content/applications/app_specific/bioinformatics_tools/de_novo_assembly_tools/trinity/running_trinity_in_multiple_steps.md +++ b/content/applications/app_specific/bioinformatics_tools/de_novo_assembly_tools/trinity/running_trinity_in_multiple_steps.md @@ -7,7 +7,7 @@ weight = "10" ## Running Trinity with Paired-End fastq data with 8 CPUs and 100GB of RAM -The first step of running Trinity is to run Trinity with the option **--no_run_chrysalis**: +The first step of running Trinity is to run Trinity with the option **--no_run_inchworm**: {{% panel header="`trinity_step1.submit`"%}} {{< highlight bash >}} #!/bin/sh @@ -21,12 +21,12 @@ The first step of running Trinity is to run Trinity with the option **--no_run_c module load trinity -Trinity --seqType fq --max_memory 100G --left input_reads_pair_1.fastq --right input_reads_pair_2.fastq --SS_lib_type FR --output trinity_out/ --CPU $SLURM_NTASKS_PER_NODE --no_run_chrysalis +Trinity --seqType fq --max_memory 100G --left input_reads_pair_1.fastq --right input_reads_pair_2.fastq --SS_lib_type FR --output trinity_out/ --CPU $SLURM_NTASKS_PER_NODE --no_run_inchworm {{< /highlight >}} {{% /panel %}} -The second step of running Trinity is to run Trinity with the option **--no_run_quantifygraph**: +The second step of running Trinity is to run Trinity with the option **--no_run_chrysalis**: {{% panel header="`trinity_step2.submit`"%}} {{< highlight bash >}} #!/bin/sh @@ -40,12 +40,12 @@ The second step of running Trinity is to run Trinity with the option **--no_run_ module load trinity -Trinity --seqType fq --max_memory 100G --left input_reads_pair_1.fastq --right input_reads_pair_2.fastq --SS_lib_type FR --output trinity_out/ --CPU $SLURM_NTASKS_PER_NODE --no_run_quantifygraph +Trinity --seqType fq --max_memory 100G --left input_reads_pair_1.fastq --right input_reads_pair_2.fastq --SS_lib_type FR --output trinity_out/ --CPU $SLURM_NTASKS_PER_NODE --no_run_chrysalis {{< /highlight >}} {{% /panel %}} -The third step of running Trinity is to run Trinity with the option **--no_run_butterfly**: +The third step of running Trinity is to run Trinity with the option **--no_distributed_trinity_exec**: {{% panel header="`trinity_step3.submit`"%}} {{< highlight bash >}} #!/bin/sh @@ -59,7 +59,7 @@ The third step of running Trinity is to run Trinity with the option **--no_run_b module load trinity -Trinity --seqType fq --max_memory 100G --left input_reads_pair_1.fastq --right input_reads_pair_2.fastq --SS_lib_type FR --output trinity_out/ --CPU $SLURM_NTASKS_PER_NODE --no_run_butterfly +Trinity --seqType fq --max_memory 100G --left input_reads_pair_1.fastq --right input_reads_pair_2.fastq --SS_lib_type FR --output trinity_out/ --CPU $SLURM_NTASKS_PER_NODE --no_distributed_trinity_exec {{< /highlight >}} {{% /panel %}} @@ -89,5 +89,5 @@ Trinity outputs number of files in its `trinity_out/` output directory after eac {{% notice tip %}} -The Inchworm (step 1) and Chrysalis (step 2) steps can be memory intensive. A basic recommendation is to have **1GB of RAM per 1M ~76 base Illumina paired-end reads**. +The Inchworm (step 2) and Chrysalis (step 3) steps can be memory intensive. A basic recommendation is to have **1GB of RAM per 1M ~76 base Illumina paired-end reads**. {{% /notice %}}