diff --git a/content/applications/app_specific/bioinformatics_tools/pre_processing_tools/scythe.md b/content/applications/app_specific/bioinformatics_tools/pre_processing_tools/scythe.md index f19e2bb045d99c632a825ba57ea68c472409e347..f24854a5d4ba3a966bdd2f7e6696921304b7ebf4 100644 --- a/content/applications/app_specific/bioinformatics_tools/pre_processing_tools/scythe.md +++ b/content/applications/app_specific/bioinformatics_tools/pre_processing_tools/scythe.md @@ -14,7 +14,7 @@ $ scythe -a adapter_file.fasta input_reads.fastq -o output_reads.fastq {{< /highlight >}} where **adapter_file.fasta** is fasta input file of the adapter sequences that need to be removed from the 3' end of the sequence data, and **input_reads.fastq** is the input sequencing data in fastq format. -The file **output_reads.fastq** contains the sequencing reads with removed adapters. If the adapter sequences are unknown, Scythe by itself provides two adapter sequences that can be used with the **-a** option: **illumina_adapters.fa** and **truseq_adapters.fasta**. +The file **output_reads.fastq** contains the sequencing reads with removed adapters. If the adapter sequences are unknown, Scythe by itself provides Illumina adapter sequences that can be used with the **-a** option: **illumina_adapters.fa**. More information about Scythe can found by typing: {{< highlight bash >}} @@ -36,13 +36,13 @@ Simple Scythe script that uses the `illumina_adapters.fa` file and `input_reads. module load scythe/0.991 -scythe -a ${SCYTHE_HOME}/illumina_adapters.fa input_reads.fastq -o output_reads.fastq +scythe -a ${SCYTHE_ADAPTERS}/illumina_adapters.fa input_reads.fastq -o output_reads.fastq {{< /highlight >}} {{% /panel %}} Scythe is single threaded program, and therefore both `#SBATCH --nodes` and `#SBATCH --ntasks-per-node` are set to **1**. -The two adapter sequences provided by Scythe are stored in **$SCYTHE_HOME**. Hence, to access the illumina adapter file use: `$SCYTHE_HOME/illumina_adapters.fa`, and to access the TruSeq file use: `$SCYTHE_HOME/truseq_adapters.fasta`. +The Illumina adapter sequences provided by Scythe are stored in **$SCYTHE_ADAPTERS**. Hence, to access the illumina adapter file use: `$SCYTHE_ADAPTERS/illumina_adapters.fa`. ### Scythe Output